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首頁   >>   公司動態   >>   推動行業轉型升級 | 百邁客攜手珀金埃爾默打造NGS全自動化實驗生產線

推動行業轉型升級 | 百邁客攜手珀金埃爾默打造NGS全自動化實驗生產線

閱讀:757      發布時間:2022-6-30
  在我國“十四五規劃”的國家戰略中,生命科學被納入引領性科技領域的重點攻關項目。其中,基因與生物技術領域重點研究包括基因組學應用、遺傳細胞和遺傳育種、合成生物、生物醫藥等技術創新與研發。這為基因測序技術在中國的發展帶來重大機遇。從全球公共衛生事件的病原追蹤、重大疾病的篩查診斷及治療的臨床需求端到基于NGS技術進行的生物物種基因組學研究、遺傳群體育種研究的科研服務端,市場需求都在不斷發展和擴容。如何推動NGS技術在科研服務領域的產業轉型升級,助力行業高質量發展成為擺在行業內企業和研究者們面前的發展戰略問題。
 
  作為中國基因測序領域的企業,百邁客(Biomarker)為了更好地滿足客戶需求,于21年9月引進explorer™ G3全自動超高通量NGS(二代測序)文庫構建系統,其高度集成化與智能化的自動化設備,在無人或人工干預極少的情況下,按照程序指令自動進行樣本制備、文庫制備、文庫質檢、信息處理等流程,配套成熟的流程控制和分析軟件,在智能化、高通量、自動化和數字化方面優勢突出,可大大提高效率,降低操作難度。
 
image.png
  百邁客生物NGS全自動化實驗生產線在2021年9月份搭建完成并通過測試;2022年1月份,產線按照規劃設計指標進行了多方位驗收,驗收結果達到合同指標要求。截至發稿,共完成微生物擴增子樣品3萬例以上,二代微生物擴增子建庫單流程通量可達1520例樣品/天;三代微生物擴增子建庫單流程通量可達1900例樣品/天;微生物擴增子建庫流程自動化占比達到70%以上,一次建庫成功率穩定在90%以上、產出波動性CV值比手工降低18%、實驗交付周期平均縮短20%以上。
 
  一套能完成擴增子建庫的全自動化實驗生產線
 
  百邁客通過大量的實驗積累和優化,力推二代微生物擴增子項目和三代微生物擴增子項目上線,目前測試成功的業務線包括:16S全長,16S V3+V4,ITS1等常見擴增區域;而普通轉錄組和重測序等項目,早期就已經在自動化液體工作站上進行大量技術積累,為順利整合到全自動化實驗生產線提供了極大助益。
 
  更智能
 
  配合LIMS系統,實現自動下單,接收訂單后自動進行時序規劃,自動啟動任務,系統記錄試劑耗材用量并進行預警提醒,真正做到無人值守,24小時不間斷運行。系統采用先進的設計理念,通過條碼管理96孔板的整個“生命周期”,每一塊板自“出生”就帶有唯一條形碼,條碼全程可追溯,通過實時甘特圖可清楚的看到該條碼所在的設備,和正在進行的實驗步驟,一系列不同條碼的孔板從自動化冰箱或儲板棧里推出,通過智能柔性機械手臂搬運到產線上各個自動化設備,最終再回到自動化冰箱或儲板棧。組成了我們的全自動化實驗標準步驟。
 
  高通量
 
  產線配套4臺PCR擴增儀,2臺核酸片段分析儀,可使用384孔模式進行檢測,預計24小時內可完成1536個樣品(16板)。然而,追求高通量遠不止如此,明年1月,將啟動通量升級,擴展到8臺PCR儀,3臺自動化片段分析儀,通量再翻倍。
 
  信息自動化
 
  LIMS與自動化系統流程控制軟件無縫銜接,LIMS下單可直接啟動系統執行生產任務,產線在實驗過程中產生的檢測結果數據,可直接回傳到LIMS。使用微流控技術進行毛細管電泳,生成電子膠圖和峰圖取代傳統電泳圖,而且可批量導入電子圖片名稱,擺脫了常規電泳制膠、染膠、照膠等繁瑣步驟。
 
  至此,百邁客貫通了從樣本提取到檢測、建庫、質檢、出報告的實驗全流程自動化,信息化。
 
  曾幾何時,我們欣喜于日益增漲的測序需求,也困惑于實驗自動化設備匱乏與滯后,今天百邁客站在了新的起點之上,結合自動化智能化為科研行業帶來更好的服務。
 
  關于百邁客生物
 
  百邁客生物(BioMarker)成立于2009年5月,是一家高新技術企業,公司基于功能基因組學的BT+IT(高通量基因測序技術、生物信息技術、高性能生物云計算技術)技術,拓展出了科技服務、生物云、智能制造三大業務板塊;擁有以博士和碩士為主體的研發、生產團隊;公司自成立以來,注重創新發展,科研成果成果層出不窮,先后在《Nature》、《Cell》、《Nature Genetics》、《Advanced Science》、《Nature Communications》等國際著名期刊上發表高質量合作文章近千篇,累計影響因子超過4500分。擁有國家發明專利技術60余項,軟件著作權近200項。公司擁有Illumina、PacBio、Nanopore、BioNano、AB SCIEIX QTRAP, Waters Q-TOF等全面的測序平臺,滿足科研工作者多種科研需求,努力打造基因產業國際一流企業,“為世界創造新的可能!”
 
  關于珀金埃爾默
 
  珀金埃爾默是知名的端到端解決方案提供商,幫助科學家、研究人員和臨床醫生更好地診斷疾病、發現新的個性化藥物、監測食品的安全與質量,并推進卓越的環境及應用分析。85年來,珀金埃爾默秉承為打造更健康的世界而持續創新的使命,不斷推動科學技術的進步。在全球,我們擁有16,000多名專業人員,與商業、政府、學術及醫療健康領域的客戶保持密切合作,為之提供涵蓋儀器、試劑、檢測方法、自動化、信息化和戰略服務的全面解決方案,助力客戶加快工作流程,制定更好的決策。珀金埃爾默于1978年進入中國,并建立了覆蓋研發、生產、銷售與服務的完整價值鏈,以先進的產品和解決方案助力中國科研及醫療健康領域的創新突破。

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